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Single Exponential Fit,
sample CFS results file (*.LST)
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Nonlinear Parameter Estimation (HETANL); 7.000 1.000: 07-Nov-96 14:13:47
Center for Fluorescence Spectroscopy --- SGI-4D70 --- USER=guest
global frequency domain HETerogenous fluorescence ANaLysis
Data input from file
COMMENTS
PPO cyc 20 ex=600.0 em=360.0
Standard Error of PHASE ANGLE set to 0.300000
Standard Error of MODULATION set to 7.000000E-03
24 data points read
GUESS
Initial value of Tau 1 is 3.00000
Initial value of Tau 2 is 0.000000E+00
Initial value of Tau 3 is 0.000000E+00
Initial value of Tau 4 is 0.000000E+00
Initial value of Tau 5 is 0.000000E+00
Initial value of Alpha 1 is 1.00000
Initial value of Alpha 2 is 0.000000E+00
Initial value of Alpha 3 is 0.000000E+00
Initial value of Alpha 4 is 0.000000E+00
Initial value of Alpha 5 is 0.000000E+00
Derived value of Norm. A 1 for ppocyc.002 is 1.00000
Derived value of Norm. A 2 for ppocyc.002 is 0.000000E+00
Derived value of Norm. A 3 for ppocyc.002 is 0.000000E+00
Derived value of Norm. A 4 for ppocyc.002 is 0.000000E+00
Derived value of Norm. A 5 for ppocyc.002 is 0.000000E+00
Derived value of S.S. Int. 1 for ppocyc.002 is 1.00000
Derived value of S.S. Int. 2 for ppocyc.002 is 0.000000E+00
Derived value of S.S. Int. 3 for ppocyc.002 is 0.000000E+00
Derived value of S.S. Int. 4 for ppocyc.002 is 0.000000E+00
Derived value of S.S. Int. 5 for ppocyc.002 is 0.000000E+00
Resulting Sum of Squares is 44789.4 with 48 observations
(Sum of Squares)/(Number of Observations) is 933.113
NonLinear Parameter Estimation (HETANL); 7.000 1.000 07-Nov-96 14:14:30
Center for Fluorescence Spectroscopy --- SGI-4D70 --- USER=guest
FIT 24 data points to 1 parameters; NDF = 47
1 + ( 1/ 47)*F-statistic( 1, 47, 33.%) = 1.0213
Maximum Fractional Change in Variance = 0.000100
Converged since variance did not change for 1 iterations
6 iterations yielded a weighted SSR of 708.949
and a weighted variance of 15.0840
for a weighted mean deviation of 3.88382
File Names: ppocyc.002 S.E.s 0.3000 7.0000E-03
Variance Co-Variance Matrix
Tau 1 ; 3.10667E-04
Eigenvalues found to be
1.00000
9 Variance Calculations in DK:HETANL.VAR
Summary of Parameters; Chi Sq. = 15.084 ; SSR = 708.95
6 Iterations; 47 Degrees of Freedom; 67.0% Confidence Intervals
Value -C.I. +C.I. Initial Guess
File = ppocyc.002 S.E.s 0.3000 7.0000E-03
Tau 1 1.6724 1.6546 1.6902 3.0000
Alpha 1 1.0000 1.0000
Norm. A 1 1.0000 1.0000 1.0000 Derived
S.S. Int. 1 1.0000 1.0000 1.0000 Derived
Asymptotic Standard Errors of Fitted Parameters
Value +/- CI/Ase
Tau 1 = 1.6724 +/- 1.76258E-02 1.0
Mean C.I./Asym.S.E. = 1.0 range of 1.0 to 1.0
NonLinear Parameter Estimation (HETANL); 7.000 1.000 07-Nov-96 14:14:30
Center for Fluorescence Spectroscopy --- SGI-4D70 --- USER=guest
Summary for File Name: ppocyc.002 S.E.s 0.3000 7.0000E-03
FREQUENCY PHASE ANGLE Calculated W. diff. MODULATION Calculated W. diff.
1 18.980 10.701 11.279 -1.9273 0.97640 0.98069 -0.61228
2 22.776 13.330 13.459 -0.43070 0.96774 0.97254 -0.68502
3 26.572 15.851 15.601 0.83516 0.95548 0.96316 -1.0971
4 34.164 20.563 19.748 2.7162 0.93151 0.94119 -1.3827
5 34.164 20.515 19.748 2.5581 0.93209 0.94119 -1.2998
6 45.552 25.748 25.578 0.56350 0.88789 0.90199 -2.0150
7 53.144 30.072 29.181 2.9715 0.85888 0.87309 -2.0297
8 60.736 33.176 32.546 2.0997 0.82787 0.84296 -2.1551
9 72.124 37.970 37.158 2.7096 0.78067 0.79698 -2.3296
10 87.308 43.381 42.534 2.8233 0.71855 0.73687 -2.6178
11 102.49 47.213 47.123 0.30184 0.65748 0.68043 -3.2788
12 121.47 51.936 51.923 4.24957E-02 0.59448 0.61671 -3.1763
13 140.45 56.218 55.880 1.1283 0.54091 0.56093 -2.8605
14 167.02 60.094 60.327 -0.77545 0.47517 0.49506 -2.8408
15 197.39 63.668 64.260 -1.9740 0.41897 0.43428 -2.1872
16 231.56 66.259 67.658 -4.6646 0.36814 0.38013 -1.7133
17 273.31 69.489 70.802 -4.3777 0.31813 0.32883 -1.5288
18 318.86 70.861 73.382 -8.4046 0.27649 0.28599 -1.3568
19 375.80 73.406 75.790 -7.9465 0.23892 0.24548 -0.93777
20 444.13 74.819 77.906 -10.290 0.20737 0.20952 -0.30691
21 520.05 77.550 79.630 -6.9337 0.17870 0.18000 -0.18632
22 614.95 79.128 81.203 -6.9179 0.15250 0.15293 -6.18781E-02
23 721.24 80.108 82.483 -7.9194 0.13018 0.13081 -9.05650E-02
24 850.30 80.120 83.614 -11.645 0.11325 0.11123 0.28921
SSR = 708.95 NPT = 48
-------- weighted residuals for PHASE ANGLE vs. log FREQUENCY ---------
11.645 | |
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| * * * * * * |
0.0 -|---*--*----------*---------------*--*-----*--------------------------------|-
|* * | | | | | | | | ||
| * * | | | | | | ||
| | | | * * | ||
| * * | * ||
-11.645 | * *|
----- SSR = 628.103 --- NPT = 24 ----------------------------------
NonLinear Parameter Estimation (HETANL); 7.000 1.000 07-Nov-96 14:14:30
Center for Fluorescence Spectroscopy --- SGI-4D70 --- USER=kazik
-------- weighted residuals for MODULATION vs. log FREQUENCY ---------
3.2788 | |
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0.0 -|-----------------------------------------------------------------*--*--*--*|-
|* * | | | | | | | | | | | | | | | | * |
| * * | | | | | | | | | | | | * * |
| * * | | | | | | | | * * |
| * * * | | | | * |
-3.2788 | * * * * |
----- SSR = 80.8464 --- NPT = 24 ----------------------------------
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