Kazimierz Nowaczyk, Ph.D.
Assistant Professor
Center for Fluorescence Spectroscopy
University of Maryland School of Medicine
Department of Biochemistry & Molecular Biology
725, West Lombard Street, Room: N263
Baltimore, MD 21201

Contact by e-mail

Area of Expertise:

  • Spectroscopy: UV-VIS, Fluorescence, Phosphorescence, Fluorescence Microscopy.
  • Instrumental Design and Development.
  • Applied Physics, Mathematics, and Statistics.
  • Data Analysis and Presentation.
  • Network and Web Sites Administration
  • Personal Computer Operating Systems and Software.
  • Personal Computer Hardware and External Devices.
  • Professional Experience:

  • Fluorescence and phosphorescence of biological systems, radiation less energy transfer, nanosecond and picosecond time-resolved spectroscopy, polarized absorption and emission in isotropic and macroscopically oriented systems, Fluorescence Lifetime Imaging Microscopy.
  • „Method and Apparatus for Multi-Dimensional Phase Fluorescence Lifetime Imaging" J.R. Lakowicz, K.W. Berndt, K. Nowaczyk, H. Szmacinski, M.L. Johnson, United States Patent Number 5485530, Jan. 16, 1996.
  • Image processing and statistical data analysis and presentation using professional, advanced software (Origin, Mathematica, Mathcad, Inovision ISee, Adobe Photoshop) and method (FFT, linear and nonlinear function fitting).
  • Network and Web Sites construction:
    - Institute of Experimental Physics in University of Gdansk,
    - Center for Fluorescence Spectroscopy.
  • PC construction and hardware upgrade, connection to the network, apparatus and external devices.
  •  Selected Publications:

    A. Kawski, K. Nowaczyk,
    „Concentration Depolarization of the Fluorescence, Delayed Fluorescence and Phosphorescence of Acridine Yellow and Proflavine in Polyvinyl Alcohol Films", Acta Physica Polonica (1990), Vol. A78, No. 3, 379-392.

    J. Rak, K. Nowaczyk, J. Blazejowski, A. Kawski,
    „Absorption and Luminescence Spectroscopic Analisis of Tautomeric Forms of N,N-Dimethyl-N'-(1-nitro-9-acridynyl)-1,3-propanediamine (Nitracrine) and Its Nitro Isomers in Poly(vinyl Alcohol) Films", Journal of Fluorescence (1991), Vol. 1, No. 1, 57-67.

    J. Rak, J. Blazejowski, K. Nowaczyk, A. Kawski,
    „Tautomerism and Stereoisomerism of Nitracrine, Its Nitro Isomers and their Protonated Forms in Solutions Examined by Absorption and Fluorescence Spectroscopy", Journal of Photochemistry and Photobiology A: Chemistry (1991), 61, 343-363.

    A. Kawski, K. Nowaczyk, B. Kuklinski,
    „Fluorescence Quantum Yields of 2-Substituded 3-Methylquinoxalines in Liquid Solution at Room Temperature", Zeitschrift für Naturforschung (1991), 46a, 700-702.

    A. Kawski, I. Gryczynski, K. Nowaczyk, P. Bojarski, J. Lichacz,
    „Deactivation of the S1-State of -Substituted 4-Dimethylamino-Trans-Styrenes in Alkaline Solutions", Zeitschirift für Naturforschung, A (1991), 46(12), 1043-1048.

    A. Kawski, I. Gryczynski, P. Bojarski, K. Nowaczyk,
    „Viscosity Independence of the Ratio of Fluorescence Lifetimes and Quantum Yields of -Substituted 4-Dimethylamino-Trans-Styrenes", Journal of Photochemistry and Photobiology A: Chemistry, (1992), 68, 185-190.

    E.M. Sevick, J.R. Lakowicz, H. Szmacinski, K. Nowaczyk, M.L. Johnson,
    „Frequency domain imaging of absorbers obscured by scattering", Journal of Photochemistry and Photobiology, B-Biology (1992), 30, 16(2), 169-183.

    E.M. Sevick, J. Frisoli, C. Burch, H. Szmacinski, K. Nowaczyk, M.L. Johnson, J.R. Lakowicz,
    „Time-Dependent Photon Migration Imaging in Two-Dimensions: A Method for Detection and Localization of Absorbers in Tissue-Like Media", Proc. Soc. Photo-Opt. Instrum. Eng. (1993), 1888, 428-439.

    E.M. Sevick, C. Burch, J. Frisoli, M.L. Johnson, H. Szmacinski, K. Nowaczyk, J.R. Lakowicz,
    „The physical basis of biomedical optical imaging using time-dependet measurements of photon migration in the frequency-domain", Medical Optical Thomography: Functional Imaging and Monitoring, Ed: I. Müller, Proc. Soc. Photo-Opt. Instrum. Eng. Optical Engineering Press, Bellingham, WA, (1994), 485-512.

    J.R. Lakowicz, H. Szmacinski, K. Nowaczyk, M.L. Johnson,
    „Fluorescence Lifetime Imaging of Free and Protein-Bound NADH", Proceedings of the National Academy of Sciences of the United States of America (1991), 89(4), 1271-1275.

    J.R. Lakowicz, I. Gryczynski, H. Szmacinski, K. Nowaczyk, M.L. Johnson,
    „Advances in Frequency-Domain Fluorometry; Gigahertz Instrumentation; Time Dependent Photo Migration and Fluorescence Lifetime Imaging", SPIE (1991), Vol. 1599 Recent Advances in the Uses of Light in Physics, Chemistry, Engineering, and Medicine, 227-243.

    J.R. Lakowicz, H. Szmacinski, K. Nowaczyk, K. Berndt, M.L. Johnson,
    „Fluorescence Lifetime Imaging", Analytical Biochemistry, (1992), 202, 316-330.

    J.R. Lakowicz, H. Szmacinski, K. Nowaczyk, M.L. Johnson,
    „Fluorescence Lifetime Imaging of Calcium Using Quin-2", Cell Calcium (1992), 13, 131-147.

    J.R. Lakowicz, H. Szmacinski, K. Nowaczyk, M.L. Johnson,
    „Fluorescence Lifetime Imaging of Ca2+ Using Visible wavelength excitation and emission", SPIE (1992), Vol. 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, 390-404.

    J.R. Lakowicz, M.L. Johnson, W.J. Lederer, H. Szmacinski, K. Nowaczyk, H. Malak, K. Berndt,
    „Fluorescence Lifetime Sensing Generates Cellular Images", Laser Focus World, May 1992, 28(5):60-0

    J.R. Lakowicz, H. Szmaciski, K. Nowaczyk, K.W. Berndt, and M.L. Johnson,
    Chapter 10. „Fluorescence Lifetime Imaging and Application to Ca2+ Imaging."
    in Fluorescence Spectroscopy, New Metods and Applications. Edited by Otto S. Wolfbeis, Springer-Verlag Berlin, Heidelberg, New York (1993), 129-147.

    H. Szmacinski, J.R. Lakowicz, W.J. Lederer, K. Nowaczyk and M.L. Johnson
    „Fluorescence Lifetime Imaging of Intracellular Calcium", Journal of Fluorescence (1993), 3(3), 161.

    J.R. Lakowicz, H. Szmacinski, K. Nowaczyk, W.J. Lederer, M.S. Kirby, M.L. Johnson,
    „Fluorescence Lifetime Imaging of Intracellular Calcium in COS Cells Using Quin-2", Cell Calcium, (1994), 15, 7-27.

    K. Nowaczyk,
    „Przestrzenne pomiary sredniego czasu zycia sensorów fluorescencyjnych", Wydawnictwo Uniwersytetu Gdanskiego, pages 105, 1996.

    J.R. Lakowicz, I. Gryczynski, K. Nowaczyk,
    „Two-Photon Excitation of Dioxane; Time-Resolved Measurements of Excited State Complex Formation with Water", Spectrochimica Acta – Part A, (1997), 53, 1637-1644.

    J. R. Lakowicz, I. Gryczynski, Z. Gryczynski, K. Nowaczyk, and C. J. Murphy,
    Time-Resolved Spectral Observations of Cadmium-Enriched Cadmium Sulfide Nanoparticles and the Effects of DNA Oligomer Binding,  (2000). Anal. Biochem. 280:128-136.

    Gryczynski I., Malicka J., Gryczynski Z., Nowaczyk K., Lakowicz J.R,
    Ultraviolet surface plasmon-coupled emission using thin aluminium films (2004). , Anal. Chem., 76, 4076-4081

    Gryczynski I., Malicka J., Nowaczyk K., Gryczynski Z., Lakowicz J.R.,
    Effects of sample thickness on the optical properties of surface plasmon-coupled emission (2004).  J. Phys. Chem. B, 108, 12073-12083

    Ignacy Gryczynski, Joanna Malicka, Kazimierz Nowaczyk, Zygmunt Gryczynski, Joseph R. Lakowicz,
    Waveguide-modulated surface plasmon-coupled emission of Nile blue in poly(vinylalcohol) thin films,  Thin Solid Films (2006), 510, 15-20.

    Single-Cell Fluorescence Imaging Using Metal Plasmon-Coupled Probe2: Single-Molecule Counting on Lifetime Image, Jian Zhang, Yi Fu, Dong Liang, Kazik Nowaczyk, Richard Y. Zhao, and Joseph R. Lakowicz, Nano Letters 8 (2008), 4, 1179-1186.